ARC Training Centre for Cryo-Electron Microscopy of Membrane Proteins for Drug Discovery (CCeMMP)

Distinct structural features of Pseudomonas aeruginosa ATP synthase revealed by cryo-electron microscopy

M Sobti, AP Gunn, SHJ Brown, L Zavan, VM Fraunfelter, AL Wolfe, CA McDevitt, PR Steed, AG Stewart

2026-12-01

F<inf>1</inf>F<inf>o</inf> ATP synthase is the ubiquitous enzyme that synthesizes cellular ATP by coupling proton-motive force wit..

ProteinDJ: A high-performance and modular protein design pipeline

D Silke, J Iskander, J Pan, AP Thompson, AT Papenfuss, IS Lucet, JM Hardy

2026-02-01

Leveraging artificial intelligence and deep learning to generate proteins de novo (a.k.a. ‘synthetic proteins’) has unlocked new f..

Bringing the ends together: Cryo-EM structures of mycobacterial Ku in complex with DNA define its role in NHEJ synapsis

J Baral, CS Ang, PJ McMillan, K Shobhana, A Saini, E Hinde, AK Das, I Rouiller

2026-01-13

Non-homologous end joining (NHEJ) is the sole pathway for repairing double-strand breaks in Mycobacterium tuberculosis during dorm..

Inhibiting heme piracy by pathogenic Escherichia coli using de novo-designed proteins

DR Fox, K Asadollahi, I Samuels, BA Spicer, A Kropp, CJ Lupton, K Lim, C Wang, H Venugopal, M Dramicanin, GJ Knott, R Grinter

2025-12-01

Iron is an essential nutrient for most bacteria and is often growth-limiting during infection, due to the host sequestering free i..

Code to complex: AI-driven de novo binder design

DR Fox, C Taveneau, J Clement, R Grinter, GJ Knott

2025-10-02

The application of artificial intelligence to structural biology has transformed protein design from a conceptual challenge into a..

Structural basis for the pore-forming activity of a complement-like toxin

BA Johnstone, MP Christie, R Joseph, CJ Morton, HG Brown, E Hanssen, TC Sanford, HL Abrahamsen, RK Tweten, MW Parker

2025-03-28

Pore-forming proteins comprise a highly diverse group of proteins exemplified by the membrane attack complex/ perforin (MACPF), ch..

Crystal structure of Alzheimer's disease phospholipase D3 provides a molecular basis for understanding its normal and pathological functions

K Ishii, SJ Hermans, ME Georgopoulou, TL Nero, NC Hancock, GAN Crespi, MA Gorman, JH Gooi, MW Parker

2024-12-01

Human 5′-3′ exonuclease PLD3, a member of the phospholipase D family of enzymes, has been validated as a therapeutic target for tr..

Lipid-Dependent Activation of the Orphan G Protein-Coupled Receptor, GPR3

Isabella C Russell, Xin Zhang, Fabian Bumbak, Samantha M McNeill, Tracy M Josephs, Michael G Leeming, George Christopoulos, Hariprasad Venugopal, Maria M Flocco, Patrick M Sexton, Denise Wootten, Matthew J Belousoff

2024-02-20

The class A orphan G protein-coupled receptor (GPCR), GPR3, has been implicated in a variety of conditions, including Alzheimer’s ..

Cryo-EM structure of the extracellular domain of murine Thrombopoietin Receptor in complex with Thrombopoietin

Kaiseal TG Sarson-Lawrence, Joshua M Hardy, Josephine Iaria, Dina Stockwell, Kira Behrens, Tamanna Saiyed, Cyrus Tan, Leila Jebeli, Nichollas E Scott, Toby A Dite, Nicos A Nicola, Andrew P Leis, Jeffrey J Babon, Nadia J Kershaw

2024-02-07

Thrombopoietin (Tpo) is the primary regulator of megakaryocyte and platelet numbers and is required for haematopoetic stem cell ma..

Xanomeline displays concomitant orthosteric and allosteric binding modes at the M4 mAChR

WAC Burger, V Pham, Z Vuckovic, AS Powers, JI Mobbs, Y Laloudakis, A Glukhova, D Wootten, AB Tobin, PM Sexton, SM Paul, CC Felder, R Danev, RO Dror, A Christopoulos, C Valant, DM Thal

2023-12-01

The M<inf>4</inf> muscarinic acetylcholine receptor (M<inf>4</inf> mAChR) has emerged as a drug target of high therapeutic interes..

Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics

Z Vuckovic, J Wang, V Pham, JI Mobbs, MJ Belousoff, A Bhattarai, WAC Burger, G Thompson, M Yeasmin, V Nawaratne, K Leach, ET van der Westhuizen, E Khajehali, YL Liang, A Glukhova, D Wootten, CW Lindsley, A Tobin, P Sexton, R Danev

2023-05-30

Allosteric modulation of G protein-coupled receptors (GPCRs) is a major paradigm in drug discovery. Despite decades of research, a..

Cholesterol-dependent cytolysins: The outstanding questions

BA Johnstone, R Joseph, MP Christie, CJ Morton, C McGuiness, JC Walsh, T Böcking, RK Tweten, MW Parker

2022-12-01

The cholesterol-dependent cytolysins (CDCs) are a major family of bacterial pore-forming proteins secreted as virulence factors by..

Dynamics of GLP-1R peptide agonist engagement are correlated with kinetics of G protein activation

G Deganutti, YL Liang, X Zhang, M Khoshouei, L Clydesdale, MJ Belousoff, H Venugopal, TT Truong, A Glukhova, AN Keller, KJ Gregory, K Leach, A Christopoulos, R Danev, CA Reynolds, P Zhao, PM Sexton, D Wootten

2022-12-01

The glucagon-like peptide-1 receptor (GLP-1R) has broad physiological roles and is a validated target for treatment of metabolic d..